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Image Search Results


PCR primers and probe.

Journal: Pathogens

Article Title: Molecular Epidemiological Study of Hand, Foot, and Mouth Disease in a Kindergarten-Based Setting in Bangkok, Thailand

doi: 10.3390/pathogens10050576

Figure Lengend Snippet: PCR primers and probe.

Article Snippet: Nested PCR was performed in a 20 μL reaction containing 10 μL of 2× nested PCR reaction mix (Vivantis, Malaysia), 6.8 μL of nuclease-free water, 0.2 μL of Taq DNA polymerase (Vivantis, Malaysia), 0.5 μL of nested primers (10 μM each) (UniEV_5UTR-F172 and UniEv_5UTR-F610, ), and 2 μL of DNA template from the first-step RT-PCR (diluted 1:50).

Techniques: Sequencing

RT-PCR and nested PCR reaction components.

Journal: Pathogens

Article Title: Molecular Epidemiological Study of Hand, Foot, and Mouth Disease in a Kindergarten-Based Setting in Bangkok, Thailand

doi: 10.3390/pathogens10050576

Figure Lengend Snippet: RT-PCR and nested PCR reaction components.

Article Snippet: Nested PCR was performed in a 20 μL reaction containing 10 μL of 2× nested PCR reaction mix (Vivantis, Malaysia), 6.8 μL of nuclease-free water, 0.2 μL of Taq DNA polymerase (Vivantis, Malaysia), 0.5 μL of nested primers (10 μM each) (UniEV_5UTR-F172 and UniEv_5UTR-F610, ), and 2 μL of DNA template from the first-step RT-PCR (diluted 1:50).

Techniques: Nested PCR, Concentration Assay